Recent Publications

Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advance fluorescence microscopy

M. Liras, S. Simoncelli, A. Rivas-Aravena, O. García, J. C. Scaiano, E. I. Alarcon, and A. Aspée, “Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advance fluorescence microscopy”, Org. Biomol. Chem., 14, 4023 - 4026 (2016).

Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advance fluorescence microscopy


A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring nitroxide depletion by hydrogen peroxide; eluding incorrect interpretation if only fluorescence intensity is considered.

http://pubs.rsc.org/en/content/articlelanding/2016/ob/c6ob00533k#!divAbstract